This group consists of high-purity fluorescent dyes produced by eliminating isomeric impurities of classical fluorescein dyes, resulting in a single, fully defined isomer.
- Advantages:
- Consistent absorption/emission spectra,
- more predictable behavior in chemical reactions,
- enhanced photostability, and low background signal.
- Chemical Properties: pH-sensitive fluorescence, high quantum yield, and suitability for functional group modification.
- Applications: High-sensitivity fluorescent assays, probe design, and biochemical analyses.